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National Centre for Cell Science
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LGC Promochem
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Biochrom
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ScienCell
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Image Search Results
Journal: AIDS Research and Human Retroviruses
Article Title: Purging Exhausted Virus-Specific CD8 T Cell Phenotypes by Somatic Cell Reprogramming
doi: 10.1089/aid.2017.0161
Figure Lengend Snippet: Establishment of B6/129OKSM mouse line. (A) Schematic illustration of B6/129OKSM mouse generation. We first established a DOX-reprogrammable fibroblast-like cell line originated from AinV15 mouse ESCs by transduction of STEM-CCA lentiviral vector encoding four DOX-inducible mouse reprogramming genes (Oct4, Klf4, Sox2, and c-Myc). Chimeric mice were generated by the injection of 8–12 established iPSCs into a B6 mouse blastocyst. The mice were bred with B6 mice to obtain F1 hybrids derived from 129P2/OlaHsd strain (agouti coat). F1 hybrids were then crossbred for >10 generations until we established a stable mouse line carrying a single copy of a reprogramming gene cassette introduced by STEM-CCA lentiviral vector homozygously and enabling induction of ESC-like colony formation from peripheral blood cells with high efficiency in the presence of DOX. DOX: doxycycline. Diff.: differentiation. (B) Fibroblasts were isolated from tail-tip biopsies of chimeric mice and exposed to 1 μg/ml of DOX for 2° iPSC induction. No 2° iPSC formation was seen with no DOX addition. (C) Expression of two pluripotent stem cell markers (Nanog and SSEA1) in 2° iPSCs. (D) Embryoid bodies generated from 2° iPSCs. iPSC, induced pluripotent stem cell.
Article Snippet:
Techniques: Transduction, Plasmid Preparation, Generated, Injection, Derivative Assay, Isolation, Expressing